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KMID : 0364820090450040419
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Korean Journal of Microbiology
2009 Volume.45 No. 4 p.419 ~ p.424
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Expression of the lux Genes in Escherichia coli for the Basis of Development of Biosensor
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Cho Mi-Mi
Kim Young-Doo
Kang Kyung-Sook
Kim Sook-Kyung
Yang In-Chul
Park Sang-Ryoul
Lee Chan-Yong
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Abstract
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To provide the basis of biosensor based on the lux genes from bioluminescent bacteria of Photobacterium leiognathi and Vibrio harveyi, we test the expression of lux genes in several strains of Escherichia coli. The expression of the recombinant plasmid of PlXba.pT7-3, containing all lux genes requiring for light emission without adding substrate, in E. coli 43R was so strong to see the blue-green light in single colony as well as in the alginate immobilized cell. In addition, the light intensity was decreased by adding heavy metal ion such as cadmium and zinc ions. These result raise the possibility that a biosensor can be developed using the lux genes system.
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KEYWORD
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bioluminescence, biosensor, Photobacterium, Vibrio
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